abstract：:A remarkable amount, of water is transported in the gastrointestinal (GI) organs to fulfil the secretory and absorptive functions of the GI tract. However, the molecular basis of water movement in the GI epithelial barriers is still poorly known. Important clues about the mechanisms by which water is transported in th...

journal_title：European journal of cell biology

authors： Calamita G,Mazzone A,Bizzoca A,Cavalier A,Cassano G,Thomas D,Svelto M

更新日期：2001-11-01 00:00:00

abstract：:Mechanical tension governs fibroblast proliferation and survival and the homeostasis of the extracellular matrix to adapt its resistance to the mechanical requirements of the organs. To consolidate this view, we analysed the effect of tension release on the expression of molecules involved in the architecture and stab...

journal_title：European journal of cell biology

authors： Lambert CA,Colige AC,Lapière CM,Nusgens BV

更新日期：2001-07-01 00:00:00

abstract：:The trafficking of intracellular membranes requires the coordination of membrane-cytoskeletal interactions. Rab proteins are key players in the regulation of vesicular transport, while Rho family members control actin-dependent cell functions. We have previously identified a rho protein, rhoD, which is localized to th...

journal_title：European journal of cell biology

authors： Murphy C,Saffrich R,Olivo-Marin JC,Giner A,Ansorge W,Fotsis T,Zerial M

更新日期：2001-06-01 00:00:00

abstract：:Expression of the Cdx1 homeobox gene in epithelial intestinal cells promotes cellular growth and differentiation. Cdx1and the Pancreatitis Associated Protein I (PAP I) are concomitantly expressed in the epithelial cells of the lower part of the intestinal crypts. Because Cdx1 is a transcription factor and PAP I, in ot...

journal_title：European journal of cell biology

authors： Moucadel V,Soubeyran P,Vasseur S,Dusetti NJ,Dagorn JC,Iovanna JL

更新日期：2001-02-01 00:00:00

abstract：:In the eye lens, three connexins have been detected in epithelial cells and bow region/differentiating fiber cells, suggesting the possible formation of heteromeric gap junction channels. To study possible interactions between Cx56 and Cx43, we stably transfected a normal rat kidney cell line (NRK) that expresses Cx43...

journal_title：European journal of cell biology

authors： Berthoud VM,Montegna EA,Atal N,Aithal NH,Brink PR,Beyer EC

更新日期：2001-01-01 00:00:00

abstract：:CD14-positive monocytes obtained from human peripheral blood were cultured with GM-CSF and IL-4. During the early culture phase immature dendritic cells (DCs) developed which not only expressed CD1a, HLA-DR and CD86, but also expressed the endothelial cell markers von Willebrand factor (vWF), VE-cadherin and VEGF rece...

journal_title：European journal of cell biology

authors： Fernandez Pujol B,Lucibello FC,Zuzarte M,Lütjens P,Müller R,Havemann K

更新日期：2001-01-01 00:00:00

abstract：:We used botulinum neurotoxins (BoNT) to examine whether differences in the secretory activity of noradrenergic and adrenergic chromaffin cells are related to differences in the exocytotic machinery of these two types of bovine adrenal medulla cells. Cleavage of syntaxin and SNAP-25 by BoNT/C1 decreased in a dose-depen...

journal_title：European journal of cell biology

authors： Baltazar G,Tomé A,Carvalho AP,Duarte EP

更新日期：2000-12-01 00:00:00

abstract：:Murine alpha1,2-mannosidase IB is a type II transmembrane protein localized to the Golgi apparatus where it is involved in the biogenesis of complex and hybrid N-glycans. This enzyme consists of a cytoplasmic tail, a transmembrane domain followed by a "stem" region and a large C-terminal catalytic domain. To analyze t...

journal_title：European journal of cell biology

authors： Becker B,Haggarty A,Romero PA,Poon T,Herscovics A

更新日期：2000-12-01 00:00:00

abstract：:Exocytosis is mediated by high-affinity interactions between different SNARE proteins. The existence of several variants of each SNARE protein suggests that the specificity of fusion may be directed by unique combination of SNARE family members. We examined if two alternatively spliced variants of synaptosomal-associa...

journal_title：European journal of cell biology

authors： Andersson J,Fried G,Lilja L,Meister B,Bark C

更新日期：2000-11-01 00:00:00

abstract：:It has been known for many years that trypanosomatids require exogenous essential growth factors in order to divide. Two surface domains are involved in starting nutrient endocytosis: the flagellar pocket and the cytostome. Although the flagellar pocket plays a fundamental role in the endocytic process occurring in se...

journal_title：European journal of cell biology

authors： Porto-Carreiro I,Attias M,Miranda K,De Souza W,Cunha-e-Silva N

更新日期：2000-11-01 00:00:00

abstract：:Tektins comprise a family of filament-forming proteins that are known to be coassembled with tubulins to form ciliary and flagellar microtubules. Recently we described the sequence of the first mammalian tektin protein, Tekt1 (from mouse testis), which is most homologous with sea urchin tektin C. We have now investiga...

journal_title：European journal of cell biology

authors： Larsson M,Norrander J,Gräslund S,Brundell E,Linck R,Ståhl S,Höög C

更新日期：2000-10-01 00:00:00

abstract：:The capacity of vascular endothelial cells to modulate their phenotype in response to changes in environmental conditions is one of the most important characteristics of this cell type. Since different growth factors may play an important signalling role in this adaptive process we have investigated the effect of endo...

journal_title：European journal of cell biology

authors： Krizbai IA,Bauer H,Amberger A,Hennig B,Szabó H,Fuchs R,Bauer HC

更新日期：2000-09-01 00:00:00

abstract：:Fas ligand can both be used by the immune system to initiate cell death, and be used by non-lymphoid cells to evade death. Recent work has shown that Fas ligand is differentially sorted in different cell types. Here we present the viewpoint that the differential sorting plays an important part in determining the role ...

journal_title：European journal of cell biology

authors： Bossi G,Stinchcombe JC,Page LJ,Griffiths GM

更新日期：2000-08-01 00:00:00

abstract：:In recent years, several antagonists of alpha(v)beta3 have been used to develop therapeutic approaches to the treatment of melanoma neoplasia. We studied the effects of anti-alpha(v)-integrin-blocking antibodies on attached M21 melanoma cells, the cellular distribution of alpha(v)-integrin and the molecular organizati...

journal_title：European journal of cell biology

authors： Castel S,Pagan R,García R,Casaroli-Marano RP,Reina M,Mitjans F,Piulats J,Vilaró S

更新日期：2000-07-01 00:00:00

abstract：:Glucocorticoid hormones regulate the transcription of nuclear genes by way of their cognate receptors. In addition, these hormones also modulate mitochondrial gene transcription by mechanisms which are as yet poorly understood. Using immunofluorescence labeling and confocal laser scanning microscopy we show that the g...

journal_title：European journal of cell biology

authors： Scheller K,Sekeris CE,Krohne G,Hock R,Hansen IA,Scheer U

更新日期：2000-05-01 00:00:00

abstract：:Proteins that are concentrated in specific compartments of the endomembrane system in order to exert their organelle-specific function must possess specific localization signals that prevent their transport to distal regions of the exocytic pathway. Some resident proteins of the endoplasmic reticulum (ER) that are kno...

journal_title：European journal of cell biology

authors： Fu J,Pirozzi G,Sanjay A,Levy R,Chen Y,De Lemos-Chiarandini C,Sabatini D,Kreibich G

更新日期：2000-04-01 00:00:00

abstract：:The central feature of fluid and electrolyte secretion by salivary acinar cells is transepithelial Cl- movement as a driving force for the secretion. However, little is known about the membrane localization and regulation by agonists of various anion channels. To characterize the anion transport and fluid secretion, w...

journal_title：European journal of cell biology

authors： Sugita M,Hirono C,Tanaka S,Nakahari T,Imai Y,Kanno Y,Shiba Y

更新日期：2000-03-01 00:00:00

abstract：:Bcl-2 can suppress apoptosis by controlling genes that encode proteins required for programmed cell death and by interference with peroxidative damage. Overexpression of Bcl-2 in NIH3T3 cells can prevent GSNO-induced (S-nitrosoglutathione-induced) apoptosis. The experimental results indicated that activation of NF-kap...

journal_title：European journal of cell biology

authors： Hour TC,Chen L,Lin JK

更新日期：2000-02-01 00:00:00

abstract：:Earlier studies have indicated that Jun/AP-1 activity is associated with, and probably required for apoptosis induction by DNA-damaging and stress-inducing agents in human myeloid cells. To investigate this possibility, we examined the capacity of continuous treatments with etoposide (10 microM) and camptothecin (0.4 ...

journal_title：European journal of cell biology

authors： Galán A,Garcia-Bermejo L,Vilaboa NE,de Blas E,Aller P

更新日期：2000-01-01 00:00:00

abstract：:Previous work has shown that Trypanosoma cruzi extracellular amastigotes as well as metacyclic trypomastigotes infect cultured cells in a highly specific parasite form-cell type interaction. In this work we have investigated the mode of interaction of both forms with HeLa and Vero cells using scanning electron and con...

journal_title：European journal of cell biology

authors： Procópio DO,Barros HC,Mortara RA

更新日期：1999-12-01 00:00:00

abstract：:Human gastric mucous cells - gastric cancer cell lines mucin gene expression - TNFalpha - RT-PCR immunocytochemistry Little is known on the expression pattern of mucin genes in human gastric cancer cell lines in relation to mucin expression in normal gastric epithelial cells. Thus, the aim of this study was to compare...

journal_title：European journal of cell biology

authors： Cornberg M,Enss ML,Makkink MK,Beil W,Bock CT,Sobek-Klocke I,Mix H,Hiller W,Manns MP,Wagner S

更新日期：1999-11-01 00:00:00

abstract：:Development of Leishmania parasites in the digestive tract of their sandfly vectors involves several morphological transformations from the intracellular mammalian amastigote via a succession of free and gut wall-attached promastigote stages to the infective metacyclic promastigotes. At the foregut midgut transition o...

journal_title：European journal of cell biology

authors： Stierhof YD,Bates PA,Jacobson RL,Rogers ME,Schlein Y,Handman E,Ilg T

更新日期：1999-10-01 00:00:00

abstract：:The presence of glutamylated tubulin, a widespread posttranslational modification of alpha- and beta-tubulin, has been investigated in Drosophila melanogaster using the specific monoclonal antibody GT335. We show here that this modification is strongly detected in brain and testis whereas other tissues analyzed did no...

journal_title：European journal of cell biology

authors： Bobinnec Y,Marcaillou C,Debec A

更新日期：1999-09-01 00:00:00

abstract：:It is generally believed that the induction of actin cytoskeleton rearrangements by extracellular stimuli results from the activation of guanine nucleotide exchange factors for the Rho GTPases. Here, we present evidence that the inactivation of RhoGAP (GTPase activating protein) activity is an equally effective means ...

journal_title：European journal of cell biology

authors： Vincent S,Settleman J

更新日期：1999-08-01 00:00:00

abstract：:The role of plasma membrane lipids in regulating the passage of ions and other solutes through the paracellular pathway remains controversial. In this study we explore the contribution of cholesterol (CH) in maintaining the barrier function of an epithelial cell line using the CH-solubilizing agent methyl beta-cyclode...

journal_title：European journal of cell biology

authors： Francis SA,Kelly JM,McCormack J,Rogers RA,Lai J,Schneeberger EE,Lynch RD

更新日期：1999-07-01 00:00:00

abstract：:Chondrocyte-matrix interaction is mediated by a series of adhesion molecules. Both alpha and beta integrin subunits are involved and govern crucial functions of cell adhesion and signal transduction. These molecules modulate proliferation and differentiation, thus establishing cartilage integrity. We studied the influ...

journal_title：European journal of cell biology

authors： Egerbacher M,Wolfesberger B,Walter I,Seirberl G

更新日期：1999-06-01 00:00:00

abstract：:Antibodies against centrin, the ubiquitous calcium-binding contractile protein, recognized a 17 kDa protein in extracts of onion root tips and cauliflower florets. Using immunofluorescence microscopy, anti-centrin antibodies were localized to the developing cell plate of onion and cauliflower root tip cells. In caulif...

journal_title：European journal of cell biology

authors： Blackman LM,Harper JD,Overall RL

更新日期：1999-05-01 00:00:00

abstract：:The hexameric ATPase p97/yeast Cdc48p has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. p97/Cdc48p contains two conserved consensus p34cdc2 kinase phosphorylation sites with...

journal_title：European journal of cell biology

authors： Mayr PS,Allan VJ,Woodman PG

更新日期：1999-04-01 00:00:00

abstract：:Intracellular free Ca2+ and H+ were quantified in Chlamydomonas reinhardtii, using the fluorescent ion indicators Fura-2 and BCECF. We demonstrate that both indicators can be loaded into living cells as acetoxymethylesters. The esters were hydrolyzed intracellularly to genuine Fura-2 and BCECF capable of indicating ch...

journal_title：European journal of cell biology

authors： Braun FJ,Hegemann P

更新日期：1999-03-01 00:00:00

abstract：:The recently established in vitro assay of condensation-sorting of pancreatic enzymes to the zymogen granule membrane (ZGM) (Dartsch, H., R. Kleene, H. F. Kern: In vitro condensation-sorting of enzyme proteins isolated from rat pancreatic acinar cells. Eur. J. Cell Biol. 75, 211-222 (1998)) was used to study the invol...

journal_title：European journal of cell biology

authors： Kleene R,Dartsch H,Kern HF

更新日期：1999-02-01 00:00:00

abstract：:A monoclonal antibody (ID5) raised against the synthetic tetradecapeptide corresponding to the C-terminal region of detyrosinated alpha-tubulin showed an unexpected cross-reactivity with beta-tubulin from pig brain tissue. The specificity and the minimal epitope requirements of ID5 were characterized by competitive en...

journal_title：European journal of cell biology

authors： Rüdiger AH,Rüdiger M,Wehland J,Weber K

更新日期：1999-01-01 00:00:00

abstract：:Fucoidan is a sulfated polysaccharide extracted from brown seaweeds. It has anticoagulant and antithrombotic properties and inhibits, as well as heparin, vascular smooth muscle cell growth. In this study, we investigated, in the presence of serum and human recombinant growth factors, the effects of fucoidan and hepari...

journal_title：European journal of cell biology

authors： Giraux JL,Matou S,Bros A,Tapon-Bretaudière J,Letourneur D,Fischer AM

更新日期：1998-12-01 00:00:00

abstract：:SNAREs are compartmentally specific membrane proteins required for intracellular membrane fusion. Homologues of the Saccharomyces cerevisiae protein Sec1p interact with, and are likely to be involved in regulation of, the syntaxin family of SNAREs. In yeast there are 7 functionally distinct syntaxins but only four cle...

journal_title：European journal of cell biology

authors： Nichols BJ,Holthuis JC,Pelham HR

更新日期：1998-12-01 00:00:00

abstract：:Etoposide induced a megabase (Mb) fragmentation pattern identical with that from genomic digestion by NotI restriction endonuclease which specifically cleaves CpG islands in euchromatin domains. Redigestion by NotI produced no change, suggesting cleavage in the same or closely related sites in euchromatin domains. Pre...

journal_title：European journal of cell biology

authors： Qi L,Sit KH

更新日期：1998-11-01 00:00:00

abstract：:In the present study, a systematic examination of the relative expression pattern of the nuclear retinoid receptors (RAR and RXR) in various liver cells was performed. Our data demonstrate that RXRalpha is the dominant receptor in all liver cells, and that RARbeta is also expressed at a high level in most cells. More ...

journal_title：European journal of cell biology

authors： Ulven SM,Natarajan V,Holven KB,Løvdal T,Berg T,Blomhoff R

更新日期：1998-10-01 00:00:00

abstract：:We examined the Ca2+ handling property and cell function of CCL39 fibroblasts highly overexpressing the cardiac isoform (NCX1) of Na+/ Ca2+ exchanger. In NCX1 transfectants in 146 mM Na+, ionomycin, alpha-thrombin or thapsigargin only produced a small transient increase in [Ca2+]i compared to the large increase seen i...

journal_title：European journal of cell biology

authors： Iwamoto T,Wakabayashi S,Imagawa T,Shigekawa M

更新日期：1998-07-01 00:00:00

abstract：:Chondrocyte terminal differentiation is associated with cellular hypertrophy increased activity of plasma membrane alkaline phosphatase and the synthesis of collagen type X. The hypertrophic phenotype of cultured chondrocytes can be stimulated by ascorbic acid but the underlying mechanisms for this phenotypic change a...

journal_title：European journal of cell biology

authors： Farquharson C,Berry JL,Mawer EB,Seawright E,Whitehead CC

更新日期：1998-06-01 00:00:00

abstract：:The major histocompatibility complex (MHC) class II-associated invariant chain (Ii) contains signals for transport to endocytic compartments where the class II molecules bind antigenic peptides for presentation to CD4+ T cells. Two leucine-based signals in the Ii cytoplasmic tail can be independently recognized for en...

journal_title：European journal of cell biology

authors： Simonsen A,Bremnes B,Nordeng TW,Bakke O

更新日期：1998-05-01 00:00:00

abstract：:Temperatures around 20 degrees C are known to block degradation of endocytosed material by preventing its transport to lysosomes, accordingly reduced temperature has been widely used to define endosomes. Newer studies have revealed that the low temperature block is proximal to perinuclear late endosomes, but it is not...

journal_title：European journal of cell biology

authors： Punnonen EL,Ryhänen K,Marjomäki VS

更新日期：1998-04-01 00:00:00

abstract：:Dendritic cells (DC) are the most effective cells for antigen presentation in primary immune responses. Human cord blood CD34+ progenitors cultured in the presence of GM-CSF and TNF alpha generate a heterogeneous population of DC including Langerhans-like DC (LLDC) and monocytes. We describe here that IL-4 exerts diff...

journal_title：European journal of cell biology

authors： Rougier N,Schmitt D,Vincent C

更新日期：1998-03-01 00:00:00